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Image Search Results
Journal: Nature protocols
Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
doi: 10.1038/nprot.2015.065
Figure Lengend Snippet: a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from T25 flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
Article Snippet: Greiner Bio-One 96-well PS uClear black TC plate, flat bottom (Greiner Bio-One, 5665-5090) MatTek glass bottomed 96-well plates (MatTek, cat. no. P96GC-0-5-F) Corning® Black/Clear bottom 96-well plates (Corning, cat. no. 3340) Lab-Tek II 8-well chambered cover glass slides (Thermo Scientific, cat. no. 155409) Lab-Tek 16-well glass chamber slides (Thermo Scientific, cat. no. 178599) MatTek glass bottomed 35 mm plates (MatTek, cat. no. P35G-0-7-C or P35G-1.5-10-C)
Techniques: Western Blot, Enzyme-linked Immunosorbent Assay