borosilicate glass-bottom slides catalog number 155409 Search Results


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Becton Dickinson t25 cell culture flasks
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
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Corning Life Sciences cell strainer filter ( nylon)
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
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GraphPad Software Inc prism for windows (version: 9.5.1)
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
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MatTek glass-bottomed 96-well plates
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
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Greiner Bio 3d cell culture greiner bio one 96 well ps uclear black tc plate
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
3d Cell Culture Greiner Bio One 96 Well Ps Uclear Black Tc Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson sorp facsaria2 cell sorter
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
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FUJIFILM human/rat aβ40 elisa kit
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
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Greiner Bio 96 well plates
a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from <t>T25</t> flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.
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a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from T25 flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.

Journal: Nature protocols

Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease

doi: 10.1038/nprot.2015.065

Figure Lengend Snippet: a. Western Blot of Aβ levels in the conditioned medium collected from undifferentiated enriched ReN-G and -GA cells. The conditioned medium was collected from T25 flask after culturing the cell for 2 days. 6E10 anti-Aβ detected ~4 kDa Aβ monomer bands in the media collected from the enriched FAD ReN cells (ReN-GA10, ReN-GA2). The 6E10 antibody also detected sAPPα (~100 kDa), which would represent the amount of full-length APPs in these cell lines. b. Increased Aβ40 and 42 levels in undifferentiated FAD ReN-GA2 cells, detected through Aβ ELISA (***, p<0.001; Student t-test; n=3 per each sample). c. Increased Aβ40 and 42 levels were also detected in a conditioned media collected from 1-week old thick-layer 3D cultures of ReN-GA2 cells (***, p<0.001; Student t-test; n=3 per each sample). The soluble Aβ levels cannot be directly compared to the levels shown in (b) since the total cell numbers of thick-layer cultures are higher than 2D undifferentiated cultures used in (b). d. Soluble Aβ levels of the various enriched FAD ReN cells. The FAD cells with PSEN1ΔE9 showed elevated Aβ42/40 ratio as compared to the enriched ReN-GA cells.

Article Snippet: Greiner Bio-One 96-well PS uClear black TC plate, flat bottom (Greiner Bio-One, 5665-5090) MatTek glass bottomed 96-well plates (MatTek, cat. no. P96GC-0-5-F) Corning® Black/Clear bottom 96-well plates (Corning, cat. no. 3340) Lab-Tek II 8-well chambered cover glass slides (Thermo Scientific, cat. no. 155409) Lab-Tek 16-well glass chamber slides (Thermo Scientific, cat. no. 178599) MatTek glass bottomed 35 mm plates (MatTek, cat. no. P35G-0-7-C or P35G-1.5-10-C) T25 cell culture flasks (BD Biosciences, cat. no. 353082) 6-well cell culture plates (Fisher Scientific/Corning life sciences, cat. no. 353046) Cell culture inserts, 0.4 μm pore size (Fisher Scientific/Falcon, cat. no. 353095) 24-well Falcon companion plates for cell culture inserts (Fisher Scientific/Falcon, cat. no. 353504) Cell culture inserts, 0.4 μm pore size (Greiner Bio-One, cat. no. 662654) ▲ CRITICAL Both cell culture inserts (Falcon and Greiner Bio-One) work well for thick-layer culture for biochemical analysis.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay